FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000827
Sequences flagged as poor quality0
Sequence length125
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167580.5584460550374947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47540.1584229947277867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45840.15275788974172785No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA160650.076.8279651
CGTACGG14200.056.8145682
GCTATCA36750.053.510051
ATCAACG251700.051.661893
CAACGCA254800.051.428275
TATCAAC241800.051.020722
TCAACGC262900.049.8437544
AACGCAG268100.049.0441676
GGTATCA60800.048.65371
GCGTACG18000.048.244161
ACGCAGA279550.046.8349767
CGCAGAG280750.046.6946568
GCAGAGT293100.044.459549
CTATCAA46200.042.928612
GCGTATG5000.031.3742661
CAGAGTA287900.031.18368710-11
AGAGTAC285950.030.78898410-11
GAGTACT97900.028.41238212-13
GCATCAA27800.028.0127371
AGTACTT101950.026.70669412-13
Produced by FastQC (version 0.11.5)