FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4716292
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA253610.5377317604592761No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC174620.3702484918236615No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC173140.367110433365873No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC142530.3022077513436403No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC139860.2965465242610084No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA134280.2847151957512385No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC129350.27426206859117286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128970.273456350879038No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT107670.22829375280410966No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA105060.22275974430760437No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG104310.2211695119810224No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG104100.22072424692957943No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC83260.176536991348288No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC77610.16455724115470374No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG76620.1624581344836155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75750.1606134649847804No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC75060.15915045124432498No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA74140.1571997662570511No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT71330.1512416958067906No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA71000.1505419935830945No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA68990.1462801709478548No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC63150.1338975618982031No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA63140.13387635880051532No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC60840.12899964633233058No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT60790.12889363084389177No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG60570.12842716269476107No Hit
GACTTAGGACGGTCAGCGTGGTCCCTTCGCCGAACACCCAAGCAGCATTC58160.12331721615201094No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC57430.12176939002080447No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT56890.12062442274566544No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT56090.11892817493064467No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG55160.116956286845683No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG54810.11621417842661141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54800.11619297532892364No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA54260.11504800805378464No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC53750.11396665007170888No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA52460.11123145046998785No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC50960.10805098581682389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47500.072.494031
GTATCAA98150.063.915491
ATCAACG132150.047.1300353
CAACGCA133400.046.7330065
TCAACGC137050.045.5317844
AACGCAG139450.045.21746
TATCAAC148600.042.3530922
CGCAGAG161200.038.7785388
ACGCAGA179600.034.7725687
GCAGAGT183250.034.0475049
TACATGG163300.030.6721442
GTACATG166250.030.4955121
AGAGTAC171650.029.3663310-11
ACATGGG176150.027.691693
GAGTACT74100.027.53148812-13
GGACGGT27750.027.22047
TAGACCG1107.7651313E-427.0393075
TCTCTGC46650.026.5195068
CTCTGCA48700.026.380239
ACGGTCA28550.026.2493299
Produced by FastQC (version 0.11.5)