FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454209
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122090.27410029479981746No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA65150.14626614961264728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63790.14321285777115533No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57440.12895667895242455No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC55000.12347871417798312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.11885387506513502No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT48540.10897557793089636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA257250.068.208721
GGTATCA166050.049.567611
CAACGCA385650.046.110135
TATCAAC388250.045.542762
ATCAACG400650.044.133233
AACGCAG409350.043.2812466
CGCAGAG409800.042.9378
TCAACGC412800.042.7809644
ACGCAGA413850.042.5873577
GCAGAGT416750.041.8927279
ATTTAGG33800.036.6345371
CTATTGA36550.033.880019
TTTAGGG38000.032.397762
ACTGTGC22450.029.5035278
TACTGTG18150.028.9573257
GGGCTAT45950.027.2613096
TAGGCAT25450.026.0251225
AGGGCTA50100.025.8658925
GTGTATG33950.025.3163281
GCTATTG50450.024.9734958
Produced by FastQC (version 0.11.5)