FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004917731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004917731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3228305
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44860.13895836979467552No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC43620.13511734486053828No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA42170.13062582376820034No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA42100.13040899171546677No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC38850.12034178926712315No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC37640.11659369235558599No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT32630.1010747125813701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.086.4716951
GTATCAA87350.065.3123251
ATCAACG119700.047.3639343
CAACGCA120500.047.0008965
TATCAAC133150.042.936582
TCAACGC134100.042.499464
AACGCAG139900.040.9093256
TTCTCTG10600.040.3701677
ACGCAGA149350.037.8052677
CGCAGAG149550.037.6378148
GCAGAGT151900.037.1355179
CTCTGCA12900.033.1759839
GTACTAG3450.031.005611
TCTAACG4450.030.7157692
AAATACG9250.030.1964875
ATTCTCT14450.030.0240156
TACTAGA7050.029.5029721
CTCACGA1454.1082494E-628.6895433
ACCGCTC850.00724934227.9706088
ACGGGCC10000.027.9369439
Produced by FastQC (version 0.11.5)