FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004917734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004917734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191808
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG121941.0231513800880678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95800.8038207496509506No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT79610.6679767210825905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68060.5710651380088068No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA44350.37212369777682314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.36197105574052196No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG41090.34477029857158203No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC38060.3193467404145634No Hit
GTACATGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT36770.30852284931801094No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT32660.2740374288475996No Hit
CTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCT31930.26791228117280635No Hit
GAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTAC30250.25381605090752873No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT26850.22528796584684782No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA26380.22134437761787132No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT24660.20691252282246808No Hit
CCCTTGCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAA24170.20280112232842876No Hit
GGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCT23580.19785066050907527No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC21830.18316708731607775No Hit
ATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCA21740.18241193212329504No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT21320.1788878745569756No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT21250.17830053162925572No Hit
GCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCTGCTATTGCAGAAT20680.1735178820749651No Hit
GGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGC20590.17276272688218236No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT20460.17167194715927397No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA20230.16974210611105145No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC19590.16437211362904092No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG19190.16101586832778433No Hit
GCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGCGAGGGT19180.16093196219525294No Hit
GTATCAACGCAGAGTACATGGGGCTCCTTGCTATAGAAGACCTGGGACAG18620.15623321877349372No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC18320.15371603479755128No Hit
GAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGC16880.1416335517130276No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG16400.1376060573515197No Hit
CTATGGAACCTGCATCTACCAGGGAAGACTCTGGGCATTCTGCTGCTGAG16080.13492106111051444No Hit
CAGCAGCAGAATGCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCG15880.13324293845988616No Hit
GCATCTACCAGGGAAGACTCTGGGCATTCTGCTGCTGAGCTTGCAGAAAA15660.13139700354419503No Hit
GCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAAAACAT15620.13106137901406936No Hit
CCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAAG15540.13039012995381807No Hit
CCTCATGGCTGGGGTCACAGATCCTCTAGCTAGGCAGGGTGACCAGAGAG15250.12795685211040705No Hit
AGCTAGGCAGGGTGACCAGAGAGGGCAGACAGCAGTCCTCTGTCCCAGGT15160.1272016969176243No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT14780.12401326388143057No Hit
GCTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACC14640.12283857802599077No Hit
GTACATGGGGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCT14540.12199951670067663No Hit
GGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACATCCCAGAAGTGGTTG14470.12141217377295672No Hit
ACTCTGGGCATTCTGCTGCTGAGCTTGCAGAAAAAGAAAAATGAGCTCAA14030.11772030394157448No Hit
TCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGGGCATTCTGCTGCT13900.11662952421866608No Hit
GAAGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTG13640.11444796477284931No Hit
GAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGAT13580.11394452797766083No Hit
GGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACA13560.113776715712598No Hit
GAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACATCCCA13510.11335718504994093No Hit
TTCTGCTGCTGAGCTTGCAGAAAAAGAAAAATGAGCTCAAAATTTGCTTT13200.11075609494146708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.11016875201374718No Hit
GCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATC13030.10932969068843304No Hit
GAGCAGATTGCAGCGGACATCCCAGAAGTGGTTGTTTCCCTTGCATGGGA13000.10907797229083879No Hit
CGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCT13000.10907797229083879No Hit
CTTTGGAGCCAAGCTTTCGTCCCATGCAAGGGAAACAACCACTTCTGGGA12970.10882625389324456No Hit
CTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAAGCT12780.10723203737514767No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG12710.10664469444742779No Hit
CTGCTGCTGAGCTTGCAGAAAAAGAAAAATGAGCTCAAAATTTGCTTTGA12570.10547000859198798No Hit
TCCTCTAGCTAGGCAGGGTGACCAGAGAGGGCAGACAGCAGTCCTCTGTC12110.10161032649554291No Hit
GTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATTCTGCAATAGCA11940.10018392224250885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)