FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004917735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004917735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191808
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG113470.9520828858339597No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT80970.6793879551068628No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA42630.3576918429814198No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG39030.3274856352701106No Hit
GTACATGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT34680.2909864676189453No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC33770.2833510095585866No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT30700.2575918268714424No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT28620.24013935130490818No Hit
CTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCT28510.2392163838470626No Hit
GAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTAC26920.2258753087745677No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA26700.2240293738588766No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT26180.21966625496724307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24510.20565393083449685No Hit
GGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCT23740.1991931586295779No Hit
CCCTTGCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAA22720.19063473311137366No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC22450.18836926753302546No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT21160.177545376436473No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT20470.1717558532918054No Hit
GCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCTGCTATTGCAGAAT20090.16856742025561164No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC19780.1659663301471378No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT19780.1659663301471378No Hit
GGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGC19630.1647077381591666No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA19420.16294570937600686No Hit
ATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCA19180.16093196219525294No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC19140.16059633766512726No Hit
GCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGCGAGGGT18480.1550585329180539No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG18000.151031038556546No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG17820.14952072817098055No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG17350.14557713994200408No Hit
GTATCAACGCAGAGTACATGGGGCTCCTTGCTATAGAAGACCTGGGACAG17080.14331167436365588No Hit
GAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGC16510.13852902480936527No Hit
TATTTGGGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGAGTAATAGCA16010.13433371818279455No Hit
GGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACATCCCAGAAGTGGTTG15550.1304740360863495No Hit
GTACATGGGGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCT14970.12560748039952746No Hit
CCTCATGGCTGGGGTCACAGATCCTCTAGCTAGGCAGGGTGACCAGAGAG14970.12560748039952746No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT14580.12233514123080227No Hit
CAGCAGCAGAATGCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCG14480.12149607990548812No Hit
GCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAAAACAT14440.12116045537536248No Hit
CTATGGAACCTGCATCTACCAGGGAAGACTCTGGGCATTCTGCTGCTGAG14310.12006967565245409No Hit
GCTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACC14010.11755249167651166No Hit
CCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAAG13810.11587436902588337No Hit
GGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACA13480.11310546665234669No Hit
GAAGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTG13280.1114273440017184No Hit
GCATCTACCAGGGAAGACTCTGGGCATTCTGCTGCTGAGCTTGCAGAAAA13190.11067218880893566No Hit
AGCTAGGCAGGGTGACCAGAGAGGGCAGACAGCAGTCCTCTGTCCCAGGT13020.10924578455590163No Hit
GAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACATCCCA12800.1073998496402105No Hit
CGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCT12740.10689641284502202No Hit
CTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAAGCT12730.1068125067124906No Hit
CTGCTGCTGAGCTTGCAGAAAAAGAAAAATGAGCTCAAAATTTGCTTTGA12670.10630906991730212No Hit
GCATTTATTTGGGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGAGTAA12590.1056378208570508No Hit
ATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGC12390.10395969820642251No Hit
GAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGAT12380.10387579207389108No Hit
GAGCAGATTGCAGCGGACATCCCAGAAGTGGTTGTTTCCCTTGCATGGGA12200.10236548168832563No Hit
TCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGGGCATTCTGCTGCT12140.10186204489313715No Hit
GTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATTCTGCAATAGCA12050.1011068897003544No Hit
ACTCTGGGCATTCTGCTGCTGAGCTTGCAGAAAAAGAAAAATGAGCTCAA11920.10001610997744602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.075.991591
GTATCAA30500.057.1171231
TATCAAC40000.043.8509252
ATCAACG40650.043.003473
CAACGCA41900.041.436745
TCAACGC43950.039.639254
AACGCAG45150.039.375876
CATGAGT14300.039.0848584
TACATGA15600.035.827792
ACATGAG15450.035.7907873
TGAGTAA15700.034.8421486
TAACGCA700.002798495633.97655
GCAGAGT53800.032.6042869
ACGCAGA54700.032.066497
GTGGTTG49000.031.7936349
AAGTGGT49150.031.6952747
CGCAGAG55600.031.654378
AGTGGTT49750.031.4325358
CAGAAGT49850.031.3694784
GGCGTCG1901.0841177E-931.2941478
Produced by FastQC (version 0.11.5)