FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004917751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004917751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551832
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG42470.2736765319957315No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT33810.21787152217508082No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA17230.11103005995494357No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA16670.10742142190649503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16450.10600374267317596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.055.1254921
GTATCAA43950.054.5436441
ATCAACG56250.041.9822733
CAACGCA57400.040.9312635
TCAACGC58350.040.5719764
TATCAAC60500.039.5247082
AACGCAG60950.038.6423456
ACGCAGA71800.032.72017
CGCAGAG72400.032.285688
GCAGAGT76150.030.773889
ACCGATC800.00537250229.7389748
GTACATG111050.028.175081
TACATGG103300.027.8703882
ACATGGG101350.027.1154123
CATGGGT13800.025.8616454
CCCGTCG1150.00100714825.8599788
AGAGTAC75850.024.34792710-11
GTATAAT7950.023.9431251
TACAGCG1751.7567349E-523.7904117
CAGAGTA78600.023.68523810-11
Produced by FastQC (version 0.11.5)