FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004917752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004917752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12561567
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT954480.7598415070349106No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA579840.46159846140214833No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG482940.3844584039554938No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT436870.3477830433098036No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT435880.3469949250758285No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT383290.3051291291922417No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG265840.211629647797922No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT261780.20839756695959985No Hit
TTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTA236470.18824880685666048No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT171130.13623300341430333No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC163720.13033405784485327No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG162130.1290682921963478No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT162100.1290444098256213No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA155520.12380620984627157No Hit
GCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATA148430.11816200956457105No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA147690.11757291108665026No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA147210.11719079315502597No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAAT144180.1147786737116476No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT138410.11018529774191388No Hit
GGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTT134380.10697709927431824No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC132600.1055600786112115No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC131860.10497098013329069No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT131700.10484360748941593No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCAT131530.104708274055299No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC128440.10224838987046761No Hit
GTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG126980.10108611449511037No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC126340.1005766239196113No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAAT125820.10016266282701831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAGT16800.062.3966031
CATGCTA115550.062.306124
TACATGC130250.055.6087842
GTACATG137900.055.255891
CAACGCA125050.054.5796175
CGCAGTA19500.054.2757532
TCAACGC126350.053.9034464
CGCAGAG128500.053.0575878
ATCAACG129100.052.8667683
AACGCAG130400.052.3955576
GGGGTTA304500.052.205666
ACATGCT140400.050.9706233
ACGCAGA137000.049.8188447
TAAGACT144600.049.3905689
GGGTTAG328600.048.4425547
TGCTAAG148050.048.386096
CCTGGGG25500.047.4318163
TAGGGGT319600.046.65244
AGGGGTT318050.046.2464945
CCTTAGC185250.045.394972
Produced by FastQC (version 0.11.5)