FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004917754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004917754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115342
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181690.3551864176432387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92320.18047669149003137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82610.16149457846611234No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA72600.14192599439099088No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG67110.1311935741539862No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC61150.11954234927009767No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC59580.11647315076880489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA319750.068.519251
ACAGAGA23650.059.966417
GGTATCA212600.047.5971531
TATCAAC476300.046.0871162
CAACGCA468900.046.0282445
ATCAACG488250.044.3823173
TCAACGC500800.043.2413524
AACGCAG501050.043.2185066
ACGCAGA502500.042.921887
CGCAGAG509350.042.4577148
GCAGAGT518150.041.639389
CAGAGAA38100.037.2232488
AATACAG40700.036.4383354
TTTAGGG35450.033.3066022
TAAAAAG26950.032.5901834
CTATTGA37150.031.5865219
TCTCTGC21300.030.756358
AAAGGTC28450.030.3649087
AGAGAAA47400.029.7680729
ATTTAGG40900.029.4325851
Produced by FastQC (version 0.11.5)