FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004917756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004917756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4827054
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205120.4249382749809718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165210.34225844583466436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78860.16337086761407685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70920.14692191137700136No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58010.1201768200645777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA88950.060.4576955
AACGCAG90800.059.3850866
GTATCAA97350.057.4568141
TCAACGC95950.056.2791064
CGCAGAG98650.054.5865828
ATCAACG102550.052.7283483
ACGCAGA102550.052.580847
GGTATCA45350.051.2974241
GTCTCGC5350.047.7085721
CTCGCTC5350.045.7577933
GCAGAGT124400.043.692619
TATCAAC134400.041.0899852
GTACTTT132550.037.1369441
CGCTCTG6100.036.5854845
TCTCGCT7600.033.158462
GAGTACG65650.032.1029741
CGGGACA4400.031.0907944
ACGGGAC7750.030.6585853
AGTACGG68900.030.514312
CGCGTAA1000.005905263629.1703821
Produced by FastQC (version 0.11.5)