FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5027092
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329930.6563038830401353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246060.4894678673077795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122420.24352050847686893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99450.1978280882864288No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90730.18048207591983595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64150.12760856574735455No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT64000.12731018250710352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95450.064.10561
ATCAACG100600.059.1201063
TCAACGC100750.058.9582674
CAACGCA103200.057.4116255
AACGCAG105350.056.2399566
GGTATCA45200.054.3752631
TCTGTCG15700.053.6489948
GTCTCGC11600.052.1831131
CGCAGAG115200.051.3057488
ACGCAGA116850.050.5812767
TATCAAC132350.045.699192
CTCGCTC12550.044.1773953
CGCTCTG12550.042.4490175
GCAGAGT140150.042.3262149
TCTCGCT14350.041.144812
GTACTTT206200.039.9314581
CTGTCGC21600.037.9959
GAGTACG47700.037.9178051
AGTACGG49650.035.675412
TCGCTCT17250.035.4785354
Produced by FastQC (version 0.11.5)