FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5027092
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162760.3237657078883776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119340.23739370594371456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59710.11877642183592424No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT56880.11314692470318825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC64950.058.530034
CAACGCA69150.056.330295
ATCAACG67200.056.1417583
AACGCAG70350.054.8577086
GTATCAA77600.048.7796671
GTCTCGC13250.047.9773251
CGCAGAG81350.047.0831078
ACGCAGA84850.045.394167
CTCGCTC14600.043.8895533
CGCTCTG13550.042.5096635
TCTGTCG17950.040.5105328
GCAGAGT92700.040.4640169
GGTATCA37400.038.8234141
TATCAAC100050.037.6401862
TCGCTCT17500.037.4392664
TCTCGCT17700.035.799472
GAGTACG48300.035.4462661
AGTACGG50600.034.010622
GTACGGG60600.030.1590141
ACGGGAC6450.030.1389433
Produced by FastQC (version 0.11.5)