FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004918430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820781
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC375560.9829403988346885No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG372890.975952298757767No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC314970.8243602551415535No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC313150.8195968311190828No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC298740.7818820288312782No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA297610.7789245183118321No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA278370.7285683215028551No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA267940.7012702376817723No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA255720.6692872478166113No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC253690.6639741979453939No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG245620.6428528617578447No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA227030.5941978878140359No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA222220.5816088386117917No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC207140.5421404681398908No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC204400.5349691594467205No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA188970.4945847458935752No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT188680.4938257387691155No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA159690.4179511989826164No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG148930.3897894173992176No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG143490.37555149065073345No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA137710.36042369348046904No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT136840.35814667210709017No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT135470.35456101776050497No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA134730.3526242409601597No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA132780.3475205723646553No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC118860.3110882303905929No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG118310.30964873412006605No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT116210.30415247563259973No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC112700.29496587216069176No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG111830.2926888507873128No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA105130.2751531689463489No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT100430.26285201899821004No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG97910.2562565088132505No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG97530.2552619477536137No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA95310.24945161735257793No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA93800.245499545773495No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA92090.2410240210051296No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG87050.22783300063521045No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC85750.22443055490487415No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA82340.21550567802760745No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC81350.21291458474065905No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT78840.2063452472151636No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC76660.2006396074519843No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA72970.19098189610972208No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT71940.18828611218491717No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC71650.18752710506045753No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT71020.18587822751421765No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT69320.18142887540531635No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA69230.18119332147013922No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG68850.18019876041050245No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA68830.18014641509157422No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA67930.17779087573980293No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC66490.17402201277696888No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC64960.17001759587895773No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA64520.16886599886253623No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC63580.16640576887290845No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA61590.1611974096395475No Hit
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC60850.15926063283920225No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA59500.15572732381154533No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT58340.15269129531370682No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT56220.14714269150731224No Hit
CATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATAT55630.14559850459892887No Hit
TAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGT55420.14504887875018221No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA54660.14305975663090872No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT54620.14295506599305222No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA53120.1390291670734334No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT52770.13811312399218903No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT52760.13808695133272492No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC52340.13698769963523166No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT52200.13662128240273388No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT51950.13596696591613075No Hit
ATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTC51900.13583610261881013No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT51180.13395167113739312No Hit
GCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAAT50260.1315437864666936No Hit
CCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGG49940.13070626136384159No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG49720.13013046285563082No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT48810.12774875084439544No Hit
CCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATA48150.12602135531976316No Hit
ATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAAAAAG48030.12570728340619367No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG47570.1245033410708439No Hit
ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT47270.12371816128692013No Hit
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG45960.12028954289711971No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT45890.12010633428087085No Hit
TTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTA44980.11772462226963545No Hit
ATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAA44870.11743672301553008No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGA42700.1117572559118149No Hit
TTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGT42640.11160021995503015No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT42540.1113384933603889No Hit
ATATGGAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAG42220.11050096825753687No Hit
ATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAG42150.110317759641288No Hit
GATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATG41580.10882591805183287No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT41360.10825011954362211No Hit
GCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTA41230.10790987497058847No Hit
CTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACT40820.10683679593255933No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC40720.10657506933791808No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC40300.10547581764042482No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA40270.10539729966203244No Hit
TTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCT40070.10487384647274993No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC40050.10482150115382169No Hit
GAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTT39280.10280620637508404No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC39090.10230892584526567No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC38730.10136671010455717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)