FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941405
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133730.6888310270139409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72840.37519219328269987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51610.2658384005398152No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC38580.19872205953935423No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33480.17245242491906634No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT32780.1688467887947131No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT32310.16642586168264736No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26200.13495380922579264No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT25630.1320177912388193No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC23020.1185739194037308No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.1172861922164618No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA20830.10729342924325423No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC20310.1046149566937347No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT19450.10018517516952928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117750.090.917631
GGTATCA50250.082.995481
TATCAAC172900.061.8681372
ATCAACG182550.058.4004863
TCAACGC183850.057.948384
CAACGCA185350.057.461655
AACGCAG187700.057.162496
ACGCAGA191650.055.7215047
CGCAGAG192050.055.567988
GCAGAGT202150.052.8272449
CAGAGTA203900.029.5396949
TCTGTCG4900.029.3718788
GTATAGA12500.024.3703611
GGGCTAT9750.024.3560646
CTGTGCA34400.024.0567659
ACTGTGC34500.023.5698668
GTATAAC3803.619789E-1022.9044761
GAGTACG194000.022.7687031
AGTACGG194100.022.6970332
TACGGGT54100.021.954824
Produced by FastQC (version 0.11.5)