FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941405
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC54250.279436799637376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38470.19815545957695585No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT29280.1508186081729469No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT27770.14304073596184205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20880.10755097468070805No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT20330.1047179748687162No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC19560.10075177513192764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101850.089.291711
GGTATCA45100.081.527871
TATCAAC149650.060.8287052
ATCAACG158550.057.3687743
TCAACGC160750.056.7626954
AACGCAG160400.056.751966
CAACGCA163250.056.2019965
ACGCAGA161550.056.080717
CGCAGAG162200.055.9447068
GCAGAGT168450.053.654019
ACTGTGC38050.032.3397148
CAGAGTA167450.031.155629
CTGTGCA37450.029.7826569
CACTGTG43300.028.4186177
ACAGCAC43000.026.7760353
AGCACTG45650.026.3251255
ACACAGC43350.025.7635861
GCACTGT46950.025.7494756
GTCTAAC6650.023.992511
GGGCTAT10900.023.7668026
Produced by FastQC (version 0.11.5)