FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702813
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573482.121789409774187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80150.053.5795631
GGTATCA44200.042.254491
CAACGCA101300.041.3863075
AACGCAG102850.040.932266
TCAACGC102650.040.8450364
ATCAACG104950.040.0117723
TATCAAC107550.039.5422252
ACGCAGA119600.035.1434637
CGCAGAG120600.034.9007038
GCAGAGT135250.031.0758029
GTACATG187700.029.5019631
TACATGG180750.029.2213422
ACATGGG177000.028.933133
TTAGACG1055.924288E-428.319784
CATGGGT36000.026.4317954
AGAGTAC129500.026.14304410-11
TATACGA2401.3083991E-824.7830222
ATACGAC2901.4733814E-1024.6121043
CATGGGA85200.024.2911914
CATGGGG64000.023.9744634
Produced by FastQC (version 0.11.5)