FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440464
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279620.43416126539951155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125140.19430277073204663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94420.14660434403483974No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC82520.1281274144223149No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT70850.11000760193675488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA359050.073.721031
GGTATCA150850.066.4608461
TATCAAC493650.053.5320362
ATCAACG507550.052.2416083
TCAACGC517250.051.4223564
AACGCAG520850.051.1798326
CAACGCA526600.050.5319025
ACGCAGA548950.048.5704547
CGCAGAG558100.047.826648
GCAGAGT592600.045.0623259
AGAGTAC573300.033.39533210-11
CAGAGTA584600.031.33187710-11
GAGTACT170600.026.62812612-13
AGTACGG548300.026.4594412-13
CGTACGG9900.025.2104072
GAGTACG554150.024.83974512-13
GCGTACG12600.024.0544681
TACGGGT129200.024.00816714-15
GCGTAAG8200.023.9164221
AGTACTT193750.023.84519412-13
Produced by FastQC (version 0.11.5)