FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440464
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC115610.17950570021041962No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT66700.10356396681978192No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA282300.074.349741
GGTATCA123400.056.5515631
TATCAAC415400.050.5258642
ATCAACG428850.048.6903843
TCAACGC431100.048.339664
AACGCAG433200.048.2034036
CAACGCA440900.047.3245625
ACGCAGA443350.046.9436577
CGCAGAG449750.046.1162648
GCAGAGT475450.043.4896439
AGAGTAC457150.034.51249710-11
CAGAGTA470400.029.25458110-11
AGTACGG552450.028.39164712-13
TACGGGC132150.025.16055514-15
TACGGGT134100.024.86121714-15
GAGTACG558600.024.48068412-13
TACGGGA206200.023.59610214-15
GTACGGG585300.022.29634514-15
TACGGGG165350.021.6734714-15
ACGGGTA32500.020.77715716-17
Produced by FastQC (version 0.11.5)