FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004918472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857138
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72360.2532604305427319Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65800.2303003915106656Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT53280.18648031701653894No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC42480.14868025275642968No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT41140.14399024478341613No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT34150.11952520319284542No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31740.11109018885332105Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA31180.10913018552131538No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA30000.10500017850030344No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGC29560.10346017588229899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)