FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705178
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC47910.2809677347467537No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT33720.19775061606471583No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA33580.19692958740964286No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT31720.18602163527795926No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC30150.17681438536035532No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG26120.15318048907504084No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT23850.13986809588207214No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC23560.13816739366799244No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG22910.13435547491229655No Hit
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGG22210.13025033163693175No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC21740.12749402115204395No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC21400.12550009441829535No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT20490.1201634081603211No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC20270.11887322027377786No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA20150.11816948142657248No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA20060.11764167729116844No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC19900.11670335882822791No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC19410.11382975853547253No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT18220.10685101496735237No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG18060.10591269650441185No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC17580.10309774111559027No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA17120.10040007553463626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78550.063.9170951
GGTATCA41750.050.0116731
TATCAAC113850.044.3081472
ATCAACG115300.043.338193
TCAACGC116400.043.030844
AACGCAG120000.041.5911986
CAACGCA120250.041.4057965
ACGCAGA123300.040.429817
CGCAGAG123900.040.4260758
GCAGAGT127250.039.361819
GGTCTAT12050.034.556661
GTCTATA11950.033.850242
ACTGTGC24150.032.268218
TCTATAC16150.032.0455323
CTGTGCA23650.031.4412369
AGAGTAC127200.030.30460510-11
TATACCC12900.029.9739785
CAGAGTA128200.027.51613410-11
ACGGACC1954.7974027E-827.4554545
TATACCG2006.132541E-826.7690685
Produced by FastQC (version 0.11.5)