FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300338
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC55390.42596617187223623No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT38160.2934621613765036No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT31590.24293683642253014No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT31150.23955310080917425No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC26820.20625406625046722No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG23960.18425978476365376No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC22780.17518522107329018No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG21950.16880226525718697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.15619015978922404No Hit
ACATATCAGCGTGCCTTCTCCCGAAGTTACGGCACCATTTTGCCTAGTTC18670.14357805432126108No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT18500.14227070192519176No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG18100.13919457864032272No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATC18030.13865625706547066No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT17440.13411897522028887No Hit
CCTTTACTATAGCTTGACACTGAACATTGAGCCTTGATGTGTAGGATAGG17220.13242710741361094No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA16940.1302738211142026No Hit
CTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCC16740.12873575947176813No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC16700.1284281471432812No Hit
GCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACT16410.1261979577617512No Hit
CCGTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACA15700.12073783893110869No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA14930.11481630160773584No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC14410.11081734133740612No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT13820.10628005949222433No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG13800.10612625332798088No Hit
GCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCT13190.10143516531855563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACA3850.094.2151264
TAACAAG7650.091.72176
CATGTAA4550.090.175642
TCATGTA4600.086.813721
AATACAG16000.082.5058064
TACAGAG15900.081.1547856
CAGAGAA16000.078.417688
ATACAGA16750.077.391495
ATAGTTC4050.076.5278851
TAGTTCA4050.076.348382
ACAGAGA16950.076.1274957
GTATCAA69800.073.693091
AGAGAAA17450.071.560839
AAAGGTC14050.071.525667
AAATACA18900.070.475433
TAAAAAG14800.070.3117454
AAGGTCA14400.068.961318
AGGTCAA14650.067.784499
CAAGCAA5700.066.76629
AACAAGC10550.065.381827
Produced by FastQC (version 0.11.5)