FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2511895
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC164100.6532916383845663No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA85670.3410572496063729No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC85130.33890747821863576No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT82500.3284372953487308No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA66850.2661337356856079No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT66310.26398396429787074No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC65330.26008252733494036No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT58120.231379098250524No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG57470.22879141046898854No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG52260.20805009763545054No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT51680.20574108392269583No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT49520.19714199837174723No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC48680.19379790954637835No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC47570.18937893502714084No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG46300.18432299120783313No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45180.17986420610734127No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC44180.1758831479819021No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA44140.17572390565688453No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC43930.17488788345054232No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG42260.1682395163810589No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG36870.14678161308494184No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA35860.1427607443782483No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT35310.14057116240925677No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT34920.13901854974033548No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG34690.13810290637148448No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC34520.1374261264901598No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA32570.12966306314555345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.12795120815161462No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG31930.12711518594527238No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG31080.12373128653864912No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC30170.12010852364449946No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA28220.1123454602998931No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG28220.1123454602998931No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC27730.11039474181842791No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA27670.11015587833090157No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC27420.10916061379954178No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA27380.10900137147452421No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG26680.10621463078671681No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC26500.10549804032413775No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT25510.10155679277995298No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGAT25140.1000838012735405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101250.080.0551761
GGTATCA59150.064.104651
AACGCAG158000.052.354586
CGCAGAG158450.051.7993858
ACGCAGA159500.051.6818287
GCAGAGT161250.050.7214859
TATCAAC161450.050.566522
ACTGTGC70450.048.398138
ATCAACG172250.047.3134463
CACTGTG66050.046.8284267
CAACGCA176700.046.61045
CTGTGCA66650.046.516649
TCAACGC178250.045.841914
TTCTCTG13500.045.2896657
TTTAGGG34700.042.49422
ACACAGC75150.041.194721
GCTATTG36800.041.0490078
CTATTGA37800.040.343669
TCTCTGC15350.039.8328868
AGCACTG82700.038.9660535
Produced by FastQC (version 0.11.5)