FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8312264
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT386450.46491545504329507No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG269560.3242919137313252No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT236190.28414641305906546No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA234300.2818726642945893No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT221880.26693088669945997No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA182510.21956713598124408No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC169150.2034944992122483No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA160540.19313631039630116No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC134100.16132788852712088No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC129730.15607059641031615No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC128820.15497582848667946No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT116560.14022653755944228No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA114230.13742345045826262No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA114030.13718284212339743No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG113130.13610010461650401No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC111300.13389853835248736No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC106500.1281239383157224No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT102750.12361253203699979No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC101290.12185609119248378No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT99930.12021995451540037No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT99830.12009965034796778No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA99830.12009965034796778No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC99670.11990716368007562No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC97720.11756123241513984No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG87060.10473680816682435No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG84080.10115174397733277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68900.070.704311
GTATCAA159450.061.775611
ATCAACG209650.046.274073
CAACGCA213400.045.399155
AACGCAG217850.044.8996666
CGCAGAG223850.043.427688
ACGCAGA223800.043.4136927
TCAACGC230450.042.148064
GCAGAGT231100.042.0128149
TATCAAC255850.038.6158332
CTCTGCA49800.035.2199069
TCTCTGC52850.033.6381578
AGAGTAC236850.032.26580410-11
TCTATAC67900.030.5727313
CAGAGTA240600.029.9482410-11
TAGGGGT178450.029.364834
ATAGGGG181800.028.9552963
TTCTCTG60250.028.7190917
GCCAAAC75000.028.6207287
TTAGCCA79200.028.5381974
Produced by FastQC (version 0.11.5)