FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004918582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865625
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT423471.0954761519805982No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA260200.6731123686337914No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG170080.43998059822150365No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT139230.3601746160064673No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT136080.35202586903799515No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG127880.3308132578819725No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT106680.27597089733225544No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA95630.24738561034761522No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA73730.1907324171382377No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA71580.18517057396928052No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC67150.17371059013742926No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT66940.17316734033953113No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT64150.1659498787388844No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC63990.16553597413096202No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC63460.1641649151172191No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA58400.1510751818916734No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT57880.14972999191592562No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC57730.1493419563459984No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA55160.14269361358124497No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT54470.14090864995957963No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC54050.13982215036378334No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT53680.1388649959579628No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT53120.13741632983023444No Hit
CTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGAT52620.13612287793047695No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG51770.13392400970088925No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT51160.13234599838318511No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT48990.12673241713823766No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT47670.12331770412287793No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG47130.12192077607113985No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT47110.12186903799514956No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT46100.11925626515763944No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT45270.11710913500404205No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG45080.1166176232821342No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT43100.11149555375909459No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC42850.11084882780921584No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC41890.10836540016168149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.10709781729991916No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT40470.10469199676637025No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTT40100.10373484236054972No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.10334680679062247No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA39940.10332093775262732No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG39620.10249312853678255No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA38710.10013904607922393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)