FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9457390
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1644141.7384711849675227No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1036461.095926043020326No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG638660.6753025940560767No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT570950.6037077883010006No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT558140.5901628250500403No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT551940.5836071051315427No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG453370.47938173216923485No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA351830.3720159578911306No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA311990.3298901705438816No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA305630.3231652707565195No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC256640.27136450965858444No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC253600.26815009215015984No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT252850.26735706151485766No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT251650.2660882124983743No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT249600.26392059542854845No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT234480.24793309782085754No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC234190.24762645930854074No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA228140.24122934551710357No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT225470.23840615645542798No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT222630.2354032137830839No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC220230.23286551575011713No Hit
TCTGAGATGTTAGTGTTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC219000.23156494550822163No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC214710.22702881027429345No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA203270.21493244965048497No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT200500.2120035231707691No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT198550.2099416435189836No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG196070.2073193555515845No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT188080.19887093585016585No Hit
CTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGAT185420.19605832053029432No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC184750.19534987982942437No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG184360.19493750389906728No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT181580.19199800367754738No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG177610.18780022818134814No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT176450.18657367413208084No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA175700.1857806434967787No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT175370.18543171001724576No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT175090.18513564524673298No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC170200.17996508550456308No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA169110.178812547647924No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT163350.17272207236880366No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT159210.1683445432619359No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT157550.1665893021224672No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT157490.16652585967164302No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATTTGT156880.1658808614215973No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC152530.16128128373684494No Hit
GGTTAGTTGTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAG151970.16068915419581933No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC151940.16065743297040727No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG151200.15987497607690915No Hit
GTGTATGGTTATCATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTGGG149120.15767563778167126No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG141670.14979820013767012No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT134470.1421851060387697No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC133830.14150838656331188No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT133560.1412228955346031No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG133490.1411488793419749No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTGTTA132170.13975314542384315No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC131540.13908699969018937No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA130480.13796618305896236No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG129370.1367924977187152No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT129020.1364224167555742No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTT128380.1357456972801164No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG128180.13553422244403582No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT122170.12917940361981478No Hit
GGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATT120910.1278471121525072No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC117580.12432605613176574No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG117470.12420974497192143No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA117010.12372335284893611No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA116900.12360704168909181No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT116400.12307835459889038No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA115660.12229589770539229No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA115140.12174606313158283No Hit
ACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATA115010.12160860448813045No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT111730.11814041717640913No Hit
CAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA109590.11587763643034707No Hit
GTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG108860.115105753278653No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC105450.1115001073234793No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC104810.1108233878480215No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG104800.11081281410621746No Hit
TTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG104440.11043215940127246No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA104380.11036871695044828No Hit
GGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTT103300.10922675283561321No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA101570.10739749550351631No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA100280.10603348281079664No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT100170.10591717165095232No Hit
GCTAAATAGGTTGTTGTTGATTTGGTTAAAAAATAGTAGAGGGATGATGC99440.10514528849925825No Hit
ATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTA99120.10480692876152933No Hit
GGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATC98920.10459545392544878No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG98720.10438397908936821No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA96940.10250185304825116No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT96640.1021846407941303No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA96270.10179341234738126No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT96190.10170882241294904No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT95740.10123300403176774No Hit
AAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTA95180.10064087449074216No Hit
GTATGGAGACATATCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTT95170.10063030074893814No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC95010.10046112088007368No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC94750.10018620359316893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG84700.0106.680681
TACATGC84700.0106.340392
CATGCTA97300.093.156744
ACATGCT101200.089.352813
TAAGACT108650.082.9618159
CGGGACA14650.076.172024
CCTGGGG9500.074.267973
CGGGGAC9600.071.994844
GGTATCA34550.071.554541
TATTAAC73700.065.160232
TGCTAAG138600.064.879846
TCCTAAA65150.063.9865152
CCCTGGG11250.062.718822
ACGGGAC25650.062.5916753
CTACTGG76950.061.937419
ACCCTGG12250.060.7799451
GGGGTTA428100.059.6353876
ACTGATC43600.059.2798463
TACTGAT44550.058.827172
CTCTAAT44300.058.2537161
Produced by FastQC (version 0.11.5)