FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004918615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4118221
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115910.28145648327275297No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG93140.22616561860084733Clontech Universal Primer Mix Long (96% over 25bp)
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG78450.19049487630702674No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA71020.17245310535787178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC68460.1662368289608547Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57240.13899205506455334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54110.13139168587601296No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC51780.1257339030615404Clontech SMART CDS Primer II A (96% over 26bp)
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA51640.12539395044607854No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGACGTTTGAGATTTGAGAG49710.12070746081863988No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC47310.1148797016964364Clontech SMART CDS Primer II A (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA45660.1108731172999215Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCA43350.10526389914480062Clontech Universal Primer Mix Long (96% over 26bp)
GTATTGGTTAGCTCAACGCCTCACAGCGCTTACACACCCAACCTATCAAC42620.10349128907846372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)