FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004918640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001486
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA171220.5704507700519009No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC149110.4967872580448484No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC127570.42502280537040654No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG123080.4100635485222986No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC112680.3754140449097547No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA109170.3637198374405211No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC105960.3530251348831879No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC105780.3524254319360477No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC102810.3425303333082347No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA94080.31344474037193576No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA91580.30511553277276654No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC84960.2830597910501665No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC83760.2790617714025652No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC83470.2780955833210616No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT76140.2536743466402975No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG73580.24514523805874824No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA72560.2417469213582872No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA71640.23868177296179294No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT66210.22059073405639743No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA58580.19516999246373296No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT56440.18804019075884412No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC54680.182176428609029No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG54060.18011078512443504No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA53470.1781450921310311No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC52610.1752798447169169No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA50550.16841657765520146No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT49900.16625098367941746No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA48690.16221964720141954No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT47150.15708885532033134No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT47080.1568556375075546No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG43080.14352890534888385No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA42390.14123004405151315No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC42280.14086355891714972No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC40950.1364324204743917No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT40600.13526633141050798No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA40290.13423350966821102No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG38490.12823648019680917No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG37170.12383865858444784No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC35080.11687544103154238No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA34890.11624242125400552No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC34570.11517628268131186No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA33690.1122444016064043No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT33320.11101167888172725No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT33270.11084509472974385No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG32410.10797984731562965No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT32400.10794653048523298No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA32180.1072135602165061No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT31840.10608078798301906No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT30800.10261583762176468No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA30350.10111658025391422No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)