FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004918644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859822
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC178310.6235003437276865No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT92560.32365650729311124No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT69290.24228780672363526No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT59670.2086493495049692No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG54450.19039646523454956No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG53810.18815856371480463No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC52740.18441707211148106No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT46600.1629472044064281No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC43040.1504988772028469No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG41880.14644268069830918No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG41840.14630281185332514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38770.13556787800079864No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT36220.12665123913306492No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA34760.1215460262911468No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG33340.11658068229421271No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG33050.11556663316807829No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG32930.11514702663312612No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC31670.11074115801612827No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA31170.10899279745382755No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC29530.10325817480948114No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG29040.10154478145842642No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC28960.10126504376845831No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA28930.10116014213472027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)