FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073883
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC31760.29574916448067434No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT21410.1993699499852405No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT18030.16789538525146594No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT14160.13185793983143415No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG12300.11453761722645764No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC12290.11444449721245238No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG12250.11407201715643138No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC11880.11062657663823713No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT11180.1041081756578696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52450.068.507081
GGTATCA25250.062.906271
TATCAAC75600.047.607752
ATCAACG79800.044.878433
TCAACGC82200.043.6404844
AACGCAG84050.043.033826
CAACGCA84750.042.6081855
CGCAGAG86100.042.07838
ACGCAGA85900.042.037767
GCAGAGT89650.040.4120679
AGAGTAC88650.031.67432210-11
GGTCTAT7450.030.3438971
GTCTATA7350.029.1379642
CAGAGTA89150.029.06102210-11
TCTATAC7700.027.0409153
ACTGTGC20100.026.637328
TACACCG3200.026.0268825
AGTACGG106900.025.84949712-13
CTGTGCA20100.024.8614989
TACGGGC21500.024.34940114-15
Produced by FastQC (version 0.11.5)