FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075748
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20390.18954253226592102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.072.611721
GTATCAA28000.060.5124631
ATCAACG36050.046.5052383
CAACGCA37800.044.3522155
TATCAAC38500.044.31792
TCAACGC38400.044.2784924
AACGCAG39200.042.919876
CGCAGAG47500.035.4201858
ACGCAGA47350.035.4068377
GCAGAGT52500.032.2763189
GGTACCG1352.3454322E-630.8263636
GTACATG71100.027.0079061
AGAGTAC50950.026.78411310-11
TACATGG70350.026.4507682
GTACTAG2308.349161E-925.8481941
CATGGGA45100.025.4412694
ACATGGG70700.025.3107553
CAGAGTA54250.024.44240210-11
AGTACAT56200.024.39067812-13
GAGTACA55150.023.61499612-13
Produced by FastQC (version 0.11.5)