FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854140
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC53800.6298733228744702No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT29130.3410447935935561No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT20220.23672934179408528No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT18740.2194019715737467No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT17050.199615988011333No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC15830.18533261526213501No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT15070.1764347765003395No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG14450.16917601329992743No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC13940.16320509518345938No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG12650.14810218465356967No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG12420.1454094176598684No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA11600.13580911794319434No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA11390.13335050460111925No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG11290.13217973634298827No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG11250.13171142903973587No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT11080.12972112300091318No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG9700.11356452103870558No Hit
GGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCA8790.10291052988971362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67050.073.434571
GGTATCA38050.055.0773121
TAACTCG400.006238346553.9565474
TATCAAC94700.052.408982
ATCAACG101150.049.21793
AACGCAG103000.048.6132776
GCAGAGT105400.047.6456349
ACGCAGA105600.047.620747
CGCAGAG105900.047.4886178
TCAACGC105150.047.4824454
CAACGCA106700.047.1972275
CTGTGCA24600.043.5772139
CACTGTG25400.043.051947
ACTGTGC26450.042.4333428
TTTAGGG5100.038.080282
ACACAGC28200.038.0576671
GCACTGT29850.037.1158376
ACAGCAC29850.036.8748253
AGCACTG30000.036.450645
CTATTGA5800.035.9731339
Produced by FastQC (version 0.11.5)