FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7607541
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC217140.2854273148182836No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG7000.048.170146
CGACGCT32850.046.696858
GTCGACG3900.045.0562176
CTAGGCG53000.042.9220127
TAGGCGG54200.041.8840838
GACGCTC37350.041.197619
CCGACGC21500.040.8652087
TCGACGC7450.040.161027
GCCGACG12050.039.8063136
GCGACAT10850.037.2059565
TGCGACA11150.036.2049034
GCTAGGC63800.036.177056
GGCTAGG69850.033.2480165
GTGCGAC7750.033.0914155
GCGACGC16050.030.181837
CTGCGAC13550.030.142723
TGGCTAG80850.029.5468544
AGGCGGG77500.029.2916479
TGTCGAC5800.028.6591515
GGCTGGC82050.028.2524381
Produced by FastQC (version 0.11.5)