FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7607541
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC13250.040.7997679
TTCCGAC15100.036.1151668
GTCCCGT7150.032.503941
ATAGCGC25950.031.4871494-95
CCGATCG26250.030.75295886-87
CGATCGG27100.029.87599486-87
GCCCCGT11500.029.2823871
CGGGGAT43050.027.9788118
GATCGGC29200.027.97057588-89
ACCCGAT29350.027.66510884-85
CGGCATA29000.027.59593890-91
GGACCGT4700.027.2465741
ATCGGCA30050.027.02137688-89
TCGGGGA44750.026.9161077
TGTCGGG10800.026.7869635
GCTCCGT15950.026.4652081
GGGCCGT10700.026.1525611
CATAGCG31400.026.0976694-95
GGTCCGT7700.025.8704831
CCCGATC31750.025.79821884-85
Produced by FastQC (version 0.11.5)