FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3824568
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT141570.3701594533029613No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG127710.3339200662663077No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA78260.2046244177120135No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA68710.17965427729354008No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC64560.16880337857765895No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA61140.15986119216601719No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT58190.1521479027173788No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT56980.14898414670624238No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT56130.1467616734752788No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55550.1452451623294448No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG52770.13797636752699913No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC52550.13740113916133795No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG52210.13651214986895252No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA50940.13319151339445395No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA49970.1306552792367661No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC49890.13044610528561656No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT49270.12882500716420783No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG48350.1264195067259884No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT47980.1254520772019219No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG46430.12139933189840002No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC46170.12071951655716412No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA45840.11985667400867235No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44420.11614383637576844No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG44020.11509796662002088No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC43140.1127970531573762No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC43080.11264017269401407No Hit
GAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTAC43040.11253558571843932No Hit
CTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCT42640.11148971596269172No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT42600.11138512898711697No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC42480.11107136806039271No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC41960.10971173737792084No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT41430.1083259599515553No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC41090.10743697065916988No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT40360.10552825835493054No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT40030.10466541580643879No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA38940.10181542072202664No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG38280.10008973562504314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77400.067.434411
GTATCAA146250.059.3313941
TATCAAC196850.044.0555532
ATCAACG199750.043.3266143
TCAACGC205700.042.2179454
AACGCAG208000.041.796286
CAACGCA208000.041.604325
ACGCAGA216350.039.935277
CGCAGAG219550.039.407378
GCAGAGT225350.038.3383339
AGAGTAC222250.030.63831910-11
CAGAGTA224850.030.13863610-11
GTACTGG23750.027.314031
TTGTAAC13550.025.8994943
TGTAACA14650.025.1728634
ACGGGTT13850.024.9067485
AGTACGG286250.023.52859712-13
GAGTACG286800.023.32802412-13
TACGGGT53400.023.0572574
TAACAAG17250.022.7514156
Produced by FastQC (version 0.11.5)