FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29524921
Sequences flagged as poor quality0
Sequence length41
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN655300.22194809598305104No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG317560.10755659600240759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC41700.027.68291923
TAACGCA44750.025.75728424
AACGCAG47850.025.00249325
CGGTAAC47250.024.4685421
GGTAACG50500.022.8942622
ACGGTAA51300.022.60477420
TCCGACC33950.021.3794639
AAGGCCG61200.021.3518735
AACGGTA55050.021.22379919
GTACGAA43200.021.21638910
CAAACGG56700.021.1304417
ATCGCGA22200.021.03595719
AAGCCCG53700.020.81743631
TCGCGAA23400.019.88244420
AAACGGT59600.019.75032618
CGAATAC46850.019.60135813
CGCAGGT58900.019.51224327
CGAGAAC230800.019.42325225
ACGAATA47800.019.24995812
GACCGTG46750.019.2304721
Produced by FastQC (version 0.11.5)