FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6563071
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTGTACTGTGTCAAATCGTTAAGAGCGAAGGAGGGACGCAGGAGGTG300240.4574687672889719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295360.45003322377588173No Hit
GTACAGGAATCTCTACCTGTTATCCATCGACTACGCCTCTCGGCCTCGCC220760.3363669233503645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131750.20074443808393966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122880.1872294235427287No Hit
TGGTAGGAGAGCGTTCTAAGAGCGGAGAAGTTGAATCGAGAGGATCAATG94110.14339323770838378No Hit
GTATCAACGCAGAGTACGGGTATGTCATCTCTGGCTAGAAGCTTTTCTTG89690.13665858559201935No Hit
TTTCTTGGCAGTGTGATTACCACACCTTCGCTACTTTTAATTCACTCCTC78300.11930390513831102No Hit
GTTTTAAAGAGCACGTGGTAGGAGAGCGTTCTAAGAGCGGAGAAGTTGAA70120.1068402276921886No Hit
GTACAGGAATCTCTACCTGTTATCCATCGACTACGCCTCTCGGCCTTGCC66800.10178162022016826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153850.077.6618351
GTATCAA433250.076.179571
TATCAAC592750.055.818162
ATCAACG624200.052.8820573
CAACGCA639650.051.6415145
TCAACGC643350.051.4187854
AACGCAG649000.050.9851846
ACGCAGA671750.049.1692967
CGCAGAG672950.049.098158
GCAGAGT713100.046.392079
AGAGTAC705050.035.99806210-11
GTACAGG124700.032.065771
CAGAGTA719000.031.24295810-11
GTACTGT129900.031.0523246
GAATCTC125000.030.4631547
TACAGGA131950.030.167222
TGTACTG134950.030.1567335
AGTACGG659750.030.10501912-13
TACTGTG136100.030.030137
CCTGTAC134150.029.7610653
Produced by FastQC (version 0.11.5)