FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004918679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103212
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG15441.4959500833236445No Hit
NAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT10881.054140991357594No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG8860.858427314653335No Hit
NGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC8120.7867302251676163No Hit
NTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT7270.7043754602178041No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT6010.5822966321745534No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4790.46409332248188195No Hit
NACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC4600.44568461031662987No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC4410.42727589815137773No Hit
NTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA4360.42243150021315345No Hit
NGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3160.3061659496957718No Hit
NGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC3130.3032593109328373No Hit
NGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT2570.24900205402472578No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC2570.24900205402472578No Hit
NGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG2520.24415765608650158No Hit
NGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA2490.24125101732356702No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA2160.20927799093128704No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC1930.18699376041545557No Hit
NGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT1660.1608340115490447No Hit
NAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT1650.15986513196139984No Hit
NCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC1610.15598961361082045No Hit
NGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC1580.1530829748478859No Hit
NTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC1530.14823857690966166No Hit
NCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1400.13564314227027865No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1340.12982986474440955No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT1310.126923225981475No Hit
NATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA1280.12401658721854047No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1270.12304770763089563No Hit
NTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC1250.12110994845560594No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1230.11917218928031623No Hit
NGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1180.114327791342092No Hit
NCCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC1170.11335891175444715No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC1120.10851451381622293No Hit
NTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT1110.10754563422857807No Hit
NCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1100.10657675464093323No Hit
NAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG1100.10657675464093323No Hit
NAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA1080.10463899546564352No Hit
NGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA1060.10270123629035383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)