FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8628226
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308620.35768650473457697No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169070.19594989746443822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155720.18047742374852027No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT150680.17463613030071304No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT121000.14023740221918155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105850.12267875227190386No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG93240.10806392878443377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153050.064.339411
ATCAACG156150.062.2463233
TCAACGC156150.062.015784
CAACGCA163200.060.617235
AACGCAG168600.057.992146
CGCAGAG173850.056.1961988
ACGCAGA178100.054.8545577
GGTATCA64500.054.1005861
GCAGAGT205350.046.69949
TATCAAC224900.043.543342
GTCTCGC13400.037.7405131
CGCTCTG16900.031.5264075
GAGTACG118650.030.7495081
CTCGCTC17500.029.210723
GGGTTAG101350.028.6271467
AGTACGG128600.028.388292
GGGGTTA108600.027.6460576
TAGGGGT101500.027.0968764
ATAGGGG101700.026.9727953
GTACGGG145950.026.3838181
Produced by FastQC (version 0.11.5)