FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004918704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001912
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT136280.3405372232073069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135490.3385631668062666No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126210.3153742511079704No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA104050.26000071965600446No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG84400.21089919018709058No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT74740.18676072837183827No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT73460.18356225724103878No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT70060.17506631829985267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66260.1655708571302917No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA58280.14563038867421374No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG56360.14083268197801452No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA52990.1324117072039565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.13073750747142865No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG50600.12643956188941685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49370.12336603103716423No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG43950.10982250484268519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)