FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004918713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288689
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA138410.4208667952488058No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC97030.2950415803987546No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC77760.23644680296616674No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC73390.22315883320070704No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC71620.21777674933689384No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA66760.20299882415150838No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT64340.19564026881228355No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA63400.19278198698630367No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC62710.19068388649702053No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG61770.18782560467104065No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT57840.17587555405816727No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC57780.17569311053736003No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA54650.16617564020191633No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC54320.16517220083747658No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT53150.16161455218173565No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC51980.15805690352599472No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG51760.15738794394970154No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG50190.15261400515524576No Hit
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC47640.1448601555209386No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT46700.1420018736949587No Hit
CCTATTATCTATCTAGAGGCTTTTCTCGGCAGTGTGAAATCAACGACTCG46370.14099843433051895No Hit
ATCCTACTGCGTCCCCCCATCGATTAAAACGATGCTAGGTGGTACAGGAA42750.12999100857514956No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG41380.1258252148500512No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT40250.12238919520818174No Hit
ACTCAAGAGAGAATATATTTTCAACTACAGGATTATTACCTTCTTTGATT40230.122328380701246No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA39940.12144657035067773No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT36310.11040873734184047No Hit
TTTCTCGGCAGTGTGAAATCAACGACTCGAGGAAACAATTTCCTCTCCCC35010.10645579439101721No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA34130.10377995608584455No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC33980.10332384728382647No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA33440.10168185559656143No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT33060.10052637996478232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)