FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004918817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666
Sequences flagged as poor quality0
Sequence length125
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191.1404561824729893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.9603841536614646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.6002400960384154No Hit
GAAGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA90.5402160864345739RNA PCR Primer, Index 38 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.42016806722689076Clontech SMART CDS Primer II A (100% over 21bp)
GAAGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA70.42016806722689076TruSeq Adapter, Index 1 (96% over 28bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60.36014405762304924No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT60.36014405762304924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60.36014405762304924No Hit
GATCGGAAGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC50.3001200480192077RNA PCR Primer, Index 31 (96% over 26bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT50.3001200480192077No Hit
GTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAA50.3001200480192077No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC50.3001200480192077No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA40.24009603841536614No Hit
CGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAA40.24009603841536614TruSeq Adapter, Index 8 (100% over 48bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC40.24009603841536614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.24009603841536614No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA40.24009603841536614No Hit
GAAGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA40.24009603841536614TruSeq Adapter, Index 11 (96% over 28bp)
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA40.24009603841536614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.24009603841536614No Hit
GAAGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAA40.24009603841536614TruSeq Adapter, Index 12 (96% over 27bp)
GAAGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA40.24009603841536614RNA PCR Primer, Index 27 (100% over 28bp)
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC40.24009603841536614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40.24009603841536614No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACC30.18007202881152462No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT30.18007202881152462No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC30.18007202881152462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTG30.18007202881152462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.18007202881152462No Hit
GAAGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA30.18007202881152462RNA PCR Primer, Index 26 (96% over 31bp)
AACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAG30.18007202881152462No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG30.18007202881152462No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG30.18007202881152462No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA30.18007202881152462No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC30.18007202881152462No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA30.18007202881152462No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA30.18007202881152462No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC30.18007202881152462No Hit
GATCGGAAGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC30.18007202881152462RNA PCR Primer, Index 1 (96% over 25bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC30.18007202881152462No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT30.18007202881152462No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC30.18007202881152462No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC20.12004801920768307No Hit
AGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCGCTGC20.12004801920768307No Hit
ATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATTAAAACCT20.12004801920768307No Hit
GGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCA20.12004801920768307No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA20.12004801920768307No Hit
ATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAAAAAG20.12004801920768307No Hit
TATCAACGCAGAGTACATGGGCTGTTAATTGTCAGTTCAGTGTTTTAATC20.12004801920768307No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12004801920768307No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA20.12004801920768307No Hit
AGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCAT20.12004801920768307No Hit
ATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGC20.12004801920768307No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT20.12004801920768307No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT20.12004801920768307Clontech SMART CDS Primer II A (100% over 26bp)
TTTCCTATTCGCCTACACAATTCTCCGATCCGTCCCTAACAAACTAGGAG20.12004801920768307No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT20.12004801920768307No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT20.12004801920768307No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT20.12004801920768307No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA20.12004801920768307No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA20.12004801920768307No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCC20.12004801920768307No Hit
GTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTAT20.12004801920768307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG20.12004801920768307No Hit
GAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAA20.12004801920768307Illumina PCR Primer Index 5 (96% over 27bp)
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG20.12004801920768307No Hit
GATCGGAAGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC20.12004801920768307RNA PCR Primer, Index 27 (96% over 27bp)
ACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTT20.12004801920768307No Hit
TCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAA20.12004801920768307TruSeq Adapter, Index 8 (100% over 46bp)
CTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGT20.12004801920768307No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA20.12004801920768307No Hit
TTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGG20.12004801920768307No Hit
CCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCT20.12004801920768307No Hit
TCCCTACTATGCCTAGAAGGAATAATACTATCGCTGTTCATTATAGCTAC20.12004801920768307No Hit
TTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGA20.12004801920768307No Hit
GTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGCCAGAGGTTACCCAAGG20.12004801920768307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT20.12004801920768307No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA20.12004801920768307No Hit
GATCGGAAGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC20.12004801920768307RNA PCR Primer, Index 46 (96% over 25bp)
GTTTGAGTTTGCTAGGCAGAATAGTAATGAGGATGTAAGTCCGTGGGCGA20.12004801920768307No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC20.12004801920768307No Hit
ATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT20.12004801920768307No Hit
GGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTTGCCACCT20.12004801920768307No Hit
AAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCCTGTCTCTT20.12004801920768307No Hit
GGGCTAGGATGATGATTAATAAGAGGGATGACATAACTATTAGTGGCAGG20.12004801920768307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC20.12004801920768307No Hit
GGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCA20.12004801920768307No Hit
CGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAG20.12004801920768307No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA20.12004801920768307No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG20.12004801920768307No Hit
GATCGGAAGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC20.12004801920768307RNA PCR Primer, Index 17 (96% over 25bp)
GCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTAAGGAGAGGAGGA20.12004801920768307No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG20.12004801920768307No Hit
GCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGG20.12004801920768307No Hit
GAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA20.12004801920768307TruSeq Adapter, Index 27 (96% over 28bp)
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA20.12004801920768307No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT20.12004801920768307No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG20.12004801920768307No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC20.12004801920768307No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA20.12004801920768307No Hit
CACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTG20.12004801920768307No Hit
ACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGC20.12004801920768307No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC20.12004801920768307No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT20.12004801920768307No Hit
GCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATA20.12004801920768307No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12004801920768307No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.12004801920768307No Hit
AGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12004801920768307No Hit
CCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT20.12004801920768307No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG20.12004801920768307No Hit
GTTCAACCTGTTCCTGCTCCGGCTTCCACTATAGCAGATGCGAGCAGGAG20.12004801920768307No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC20.12004801920768307No Hit
GGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATG20.12004801920768307No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC20.12004801920768307No Hit
GTATCAACGCAGAGTACATGGGATTGAAGAGTTTGATCATGGCTCAGATT20.12004801920768307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20.12004801920768307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.12004801920768307No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG20.12004801920768307No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC20.12004801920768307No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA20.12004801920768307No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA20.12004801920768307No Hit
TCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGAC20.12004801920768307No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT20.12004801920768307No Hit
CAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTC20.12004801920768307No Hit
CCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATT20.12004801920768307No Hit
CCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGA20.12004801920768307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATG20.12004801920768307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)