FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004918818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666
Sequences flagged as poor quality0
Sequence length125
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT40.24009603841536614No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA30.18007202881152462No Hit
GTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCT30.18007202881152462No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC30.18007202881152462No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA30.18007202881152462No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG20.12004801920768307No Hit
ATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGT20.12004801920768307No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA20.12004801920768307No Hit
ACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCT20.12004801920768307No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA20.12004801920768307No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAAGCCGGAGCAGGAACAGG20.12004801920768307No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA20.12004801920768307No Hit
GTTCATTATAGCTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCA20.12004801920768307No Hit
GTACATGGGAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAG20.12004801920768307No Hit
GGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTT20.12004801920768307No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA20.12004801920768307No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC20.12004801920768307No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT20.12004801920768307No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTCTGTCTCTT20.12004801920768307No Hit
CGACAGCCATGCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCATC20.12004801920768307No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA20.12004801920768307No Hit
CCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAATCAAAGAC20.12004801920768307No Hit
ACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAA20.12004801920768307No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC20.12004801920768307No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT20.12004801920768307No Hit
ATTCGGAAATCACCGGTTATAACGGTTCATATCACCTTACCGACGCTTAT20.12004801920768307No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA20.12004801920768307No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT20.12004801920768307No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAG20.12004801920768307No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG20.12004801920768307No Hit
CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG20.12004801920768307No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG20.12004801920768307No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT20.12004801920768307No Hit
GACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGG20.12004801920768307No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTG20.12004801920768307No Hit
AATTAGAATTGTGAAGATGATAAGTGTATGAGTAGGTGGCCTGCAGTAAT20.12004801920768307No Hit
AACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATT20.12004801920768307No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT20.12004801920768307No Hit
CGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGC20.12004801920768307No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20.12004801920768307No Hit
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT20.12004801920768307No Hit
GGGCTAGGCCCACCGCTGCTTCGCAGGCGGCAAAGACTAGTATGGCAATA20.12004801920768307No Hit
TATTAACCATTTCCTTAAATTCTTCTGGGTCTGCTGACAAAGCATGATCA20.12004801920768307No Hit
CAATATAAAAATTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCC20.12004801920768307No Hit
AAACAGCAAGCTGTTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTG20.12004801920768307No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC20.12004801920768307No Hit
CAATTATATTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGA20.12004801920768307No Hit
AGGTTAAGGAGGGTGATGGTGGCTATGATGGTGGGGATGATGAGGCTATT20.12004801920768307No Hit
TTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTAC20.12004801920768307No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC20.12004801920768307No Hit
ATATGGAGGATGGGGATTATTGCTAGGATGAGGATGGATAGTAATAGGGC20.12004801920768307No Hit
CCTTTTACCATCATTGGACAAGTAGCATCCGTACTATACTTCACAACAAT20.12004801920768307No Hit
GTAGAACTGCTATTATTCATCCTATGTGGGTAATTGAGGAGTATGCTAAG20.12004801920768307No Hit
GTATCAACGCAGAGTACATGGGACTGGACTCGCAACGTGGGTCCATCAGT20.12004801920768307No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC20.12004801920768307No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG20.12004801920768307No Hit
TCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGA20.12004801920768307No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA20.12004801920768307No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT20.12004801920768307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)