FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10643310
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1242141.1670617505268568No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1062840.9985991200105981No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC784530.7371109175622997No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA752000.7065471173911123No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC748490.7032492711383959No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG635680.5972578079563594No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC564550.5304270945786602No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA558060.524329367461814No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC525270.49352128238301807No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC523450.49181128802975765No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC504140.4736684358531322No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC501660.4713383336574806No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT493590.46375610594824357No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA474960.44625215276074837No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA373240.3506803804455569No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA372340.34983477884229625No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA356350.33481125702436554No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG348980.32788671945099784No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA310750.2919674424591598No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA303790.2854281233939442No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC298850.2807867101493802No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT298100.280082042146663No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC292990.2752809041548165No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG288230.27080861123090466No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA284370.2671819199102535No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT283280.26615780241297116No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT283220.2661014289727538No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT268520.2522899361194967No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA265450.2494054950950409No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT257290.24173870722547777No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA253070.23777377526352234No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC250600.23545306864124035No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA246530.23162907027982835No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC244810.23001303166026357No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG242220.22757957815754687No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA235620.2213784997336355No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT226920.21320435090211595No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC221390.2080085988287478No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT216730.20363026163853162No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA215780.20273768216842314No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG214580.20161021336407564No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT213530.20062367816027155No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA210860.19811506007059834No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG201360.1891892653695138No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA198160.18618268189125375No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT196380.18451026983147162No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG192050.18044198656245097No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA190940.17939907791842952No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG188290.1769092509754954No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG186970.17566903529071312No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG179220.16838746592930207No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC177300.16658351584234604No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC177120.1664143955216939No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT172240.16182935571734733No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA169130.15890733239941335No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG162770.15293174773637147No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT161950.15216131072006733No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA152030.14284090193746118No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT151410.1422583763885483No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT150880.14176041099996148No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT149730.14067992006246177No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA147080.13819009311952765No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG146000.1371753711956149No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC145420.13663042794018027No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA145360.13657405449996288No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA140110.13164137848094248No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT139130.13072061229072535No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG137810.12948039660594307No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC136910.12863479500268243No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG135590.12739457931790016No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA132840.12481079664127043No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC131270.1233356916222491No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG131100.12317596687496653No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT130490.1226028368994232No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT129690.1218511910298582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129590.12175723529616257No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA128790.12100558942659756No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT125600.11800840152170705No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA123970.11647692306246836No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG123900.11641115404888142No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA123430.11596956210051196No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT121480.11413742529344725No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG120110.11285023174181716No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA119450.11223012389942602No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA118580.11141270901627406No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA118450.11129056656246974No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC117580.1104731516793178No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG114520.10759810622823164No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC112420.10562503582062348No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC110470.10379289901355875No Hit
CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA110390.10371773442660226No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC109140.10254328775540691No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT108750.10217686039399397No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT108700.10212988252714615No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT108440.10188559761953753No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC108420.1018668064727984No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA107340.10085208454888564No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT107320.10083329340214653No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT106720.10026955899997275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA227800.091.59541
GGTATCA103200.081.409911
TTCTCTG147850.061.8993537
TCTCTGC154700.059.0654078
ATCAACG364050.058.208313
CGCAGAG363250.058.0796368
AACGCAG366200.057.8903056
ACGCAGA367400.057.4431887
CAACGCA375750.056.533965
TCAACGC380250.055.6526834
GCAGAGT377050.055.4927339
TATCAAC389000.053.97312
TATTCTC178950.052.754245
CTCTGCA183500.050.2634749
ATTCTCT206750.045.7652936
ACTGTGC94750.042.1009988
CTGTGCA93150.040.580369
TTGCCTC32950.039.769989
CACTGTG97700.038.9135867
TCTCACA49850.038.2754362
Produced by FastQC (version 0.11.5)