FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4077083
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43910.10769954891769434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140350.070.508781
GGTATCA58600.060.521821
ATCAACG197400.049.5609173
TCAACGC199050.049.1201934
CAACGCA199850.048.953345
TATCAAC202650.048.2475852
AACGCAG205500.047.6086
ACGCAGA207350.047.038017
CGCAGAG214900.045.330068
GCAGAGT228350.042.6809049
AGAGTAC217700.034.7245810-11
AGTACGG245350.030.09417512-13
CAGAGTA222950.029.49005110-11
TACGGGT47700.026.13074114-15
GAGTACG248450.025.76736512-13
TACGGGC70150.024.25629414-15
GTACGGG261100.023.97148714-15
TACGGGG88950.023.51069514-15
TACGGGA95150.023.229314-15
GTATATA24850.019.1569771
Produced by FastQC (version 0.11.5)