FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004918826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3445008
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA107380.3116973893819695Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT99680.2893462076140317Clontech Universal Primer Mix Long (96% over 26bp)
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT62630.18179928754882427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48430.14058022506769216No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG45440.1319009999396228Clontech Universal Primer Mix Long (96% over 26bp)
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG43040.12493439783013566No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG38290.11114633115510908Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT37130.10777914013552364No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC36190.10505055430930785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)