FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771647
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92150.33247379626626333No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT68010.24537756792261065No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA51210.18476378846223923No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC50050.18057855130902312No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA48570.17523876597560945No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA43810.1580648617951709No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG40730.14695233556076948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38640.1394116927588542No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC36530.13179889069567663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.12494376087575365No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC34460.1243304071550237No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT30900.11148605865032596No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG29900.10787809558720862No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT29020.10470308809166534No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC28990.10459484919977183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97650.065.914551
GGTATCA44150.051.066351
ATCAACG128800.049.693453
CAACGCA130750.048.9978265
TCAACGC131850.048.5890434
AACGCAG134700.047.6493156
ACGCAGA141050.045.3776447
CGCAGAG142850.044.680948
GCAGAGT155300.041.32889
TATCAAC170800.037.4737552
AGAGTAC155950.031.60166210-11
CAGAGTA159100.027.14371110-11
AGTACGG128650.025.9160412-13
CTAACGC3101.2732926E-1124.9450533
CGGGACG1101.5448313E-724.3345116-17
TACGGGT26950.024.2793314
AGTACTT66650.024.18643812-13
GAGTACG126950.023.3826711
ACGGGAC7000.022.0941893
TACGGGG52450.021.77501914-15
Produced by FastQC (version 0.11.5)