FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771647
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT64260.23184770643592062No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA53660.19360329796687675No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA47120.1700072195340893No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC46950.16939386581335936No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG41750.15063245788514915No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA41080.14821512263286052No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC35420.12779405169561636No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC31220.11264060683052352No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT30380.10960991785750494No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG28610.10322382323578726No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC28200.10174455837990914No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG28050.10120336392044153No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC27720.1000127361096128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84500.059.955531
ATCAACG105500.047.4569633
TCAACGC107950.046.4901924
CAACGCA107800.046.3323065
AACGCAG109150.045.7394336
ACGCAGA110500.045.0729267
CGCAGAG112150.044.463658
GCAGAGT122100.041.0352259
GGTATCA40150.040.9241751
TATCAAC149500.033.68882
GTACGAC750.003900887431.7508343
AGAGTAC125700.029.42159510-11
AGTACGG139600.026.34217612-13
TACGGGT28450.026.03921314-15
CAGAGTA128350.026.00922210-11
GTCGTGC1951.4590296E-624.423721
GAGTACG139000.023.9301612-13
GTACGGG146400.022.06985714-15
TACGGGA53900.021.4167614-15
TATACCG7600.021.1398896
Produced by FastQC (version 0.11.5)