FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396468
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG164630.48470940989286515No Hit
GATTTACCTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAA157790.46457084241629837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156200.4598895087484999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62000.18254257069402685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58760.17300324925775837No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTATTTGGGTT54040.15910646000492276No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACATGGGTT53880.15863538240313174No Hit
AGTTCGAGGAGCTTGTAGAACTTGCATGCAGTAATAAACCCCAACATCCT52700.15516118508992283No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG52010.15312966293219898No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51830.15259970063018408No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT51620.15198141127783332No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA47150.13882068077779622No Hit
ATTTAAAGGAGTTTGTAGAGCCTGCATGCAGTAATAAATCCCAACATCCT44310.13045905334600533No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44170.13004686044443817No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT43300.1274853759846994No Hit
GGATACAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACA41940.1234812163694756No Hit
CCTCGAACTTTCGGCCCTGGGACCAGACTGGATATCAACCGAACTGTGGC39900.11747497694663987No Hit
TCCACTTTGGTCCCAGGGCCGAAAGTTCGAGGAGCTTGTAGAACTTGCAT37140.10934888831574446No Hit
GGATTTACCTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACA36880.10858338721283405No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA35750.10525640165018485No Hit
TCCACTTTGGTCCCAGGGCCGAAATTTAAAGGAGTTTGTAGAGCCTGCAT35410.10425536174637888No Hit
GGATTTACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA34850.10260659014011024No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG34610.1018999737374237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113200.071.9466251
GGTATCA40350.069.295941
TTACCTA64800.059.576094
ACCTATT71300.054.3117456
TTTACCT71500.054.1598243
GATTTAC73300.053.2418751
ATCAACG152900.053.1429333
CAACGCA155700.052.378275
TCAACGC156150.052.151144
AACGCAG160800.050.9389726
TACCTAT79350.048.8018575
CGCAGAG169500.048.4647838
CCTATTT89200.043.412867
ACGCAGA191500.042.8038447
ACGGGAC20300.041.6097033
TATCAAC196200.041.5662422
TCCGAGA6950.040.2267843
GTACGGG197100.039.509951
ATTTACC101850.039.3641782
ACGGGGC17550.037.9614873
Produced by FastQC (version 0.11.5)