FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004918857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477100
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330871.3357151507811555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194720.7860804973557789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130490.5267853538411853No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55760.22510193371280932Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT48470.19567235880666908No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48330.195107181785152Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38630.15594848815146745No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT34180.13798393282467403No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT33030.1333414072907836No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.1219571272859392Clontech SMART CDS Primer II A (100% over 21bp)
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA30120.12159379920067821No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGC27930.11275281579266078No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC25250.1019337128093335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)