FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376963
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA49570.2085434228467166No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT41530.17471874825144523No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC40930.1721945188040369No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38890.16361213868284868No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT38340.16129826168939104No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC37260.15675464868405609No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC35770.15048614555632545No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC35540.14951852426815226No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA35140.14783570463654672No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG35100.14766742267338617No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA33120.13933746549693876No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG32370.13618217868767837No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT30860.12982953457836743No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG28840.12133129543875946No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA28510.11994296924268488No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT28180.11855464304661031No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG27180.11434759396759646No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA26960.11342204317021341No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA26310.11068746126885441No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA26290.11060332028727414No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG25840.1087101482017179No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA25300.10643834169905043No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG24820.10441895814112379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.079.5827941
GTATCAA79900.062.3218541
ATCAACG109550.045.1265373
TATCAAC111500.044.6059842
CAACGCA114400.043.2544565
AACGCAG115050.043.2086756
TCAACGC115250.042.9453934
ACGCAGA116650.042.6669857
CGCAGAG117200.042.513028
GCAGAGT122200.040.7240229
AGAGTAC121650.032.36985410-11
CAGAGTA123350.032.14041510-11
GTACTGG18500.026.6911961
ACGGGTA5250.026.02003516-17
TACGGGT24100.025.75364114-15
GAGTACG154300.025.46263312-13
AGTACGG154950.025.20249612-13
ACGGGTT9550.024.9109445
GACCGTT7650.023.3190617
GTAGCGA6100.022.4178719
Produced by FastQC (version 0.11.5)